Coding
TCepsE

Part:BBa_K2471002

Designed by: Armando Palacios Chaparro   Group: iGEM17_Tec-Chihuahua   (2017-10-24)

(T7 promoter + RBS) + (epsE) + (T1 terminator)


This BioBrick™ contains the necessary genetic circuitry to constitutively express the epsE gene, that when expressed, results in the production of the epsE enzyme. This will cause the disengagement of the flagellum from the motor proteins in the cell membrane, promoting biofilm formation and inhibiting motility.


A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 800
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None